Contact person
Szilárd Póliska
- E-mail: poliska@med.unideb.hu
- Phone: +36 52 411 717 /50216, 50210
Next generation sequencing (NGS) service
NGS is a high-throughput technology that enables rapid sequencing of various DNA or RNA samples. With its high throughput and speed, NGS enables researchers to carry out a wide variety of applications including gene expression profiling, detection of epigenetic changes and metagenome or whole genome sequencing. NGS is driving genomic and transcriptomic discoveries and enabling the future of personalized medicine.
We offer NGS sequencing services on Illumina MiSeq or NextSeq 500 instruments.
Instrumentation
Illumina NextSeq 500
This NGS machine is suitable to sequence single or paired-end reads, and flow cells are available in configurations for high output and mid output. With tunable output and high data quality, this instrument is suitable for many NGS based applications.
Performance parameters:
|
Flow cel configuration |
Read length |
Output (Gb/run) |
Run-time (hour) |
Data quality |
|---|---|---|---|---|
|
High-output flow cel (400 M single read, 800 M paired-end read) |
1 x 75 bp |
30 |
11 |
> 80% > Q30 |
|
2 x 75 bp |
60 |
18 |
> 80% > Q30 |
|
|
2 x 150 bp |
120 |
29 |
> 75% > Q30 |
|
|
Mid-output flow cell (130 M single read, 260 M paired-end read) |
2 x 75 bp |
19 |
15 |
> 80% > Q30 |
|
2 x 150 bp |
39 |
26 |
> 75% > Q30 |
Illumina MiSeq
This benchtop NGS machine is suitable to generate 15 Gb of output with 25 million reads and 2×300 bp read lengths. With tunable output and high data quality, this instrument can be used for several NGS based applications such as targeted genome sequencing, metagenomics and small genome sequencing.
We can provide the following NGS applications (from library preparation to FASTQ files):
1. Whole genome sequencing
2. Whole exome or target genome sequencing
3. mRNA sequencing (polyA selection)
4. Stranded RNA sequencing (depletion of ribosomal RNA)
5. microRNA sequencing
6. ChIP-Seq
7. Metagenomic sequencing
- 16S V3-V4 region sequencing
- shotgun sequencing
8. Amplicon sequencing
Please contact us regarding your research project so we can discuss different NGS methods that could be applicable to your genomic experiments.
NGS data storage and transfer
After the sequencing reaction we employ demultiplexing and provide FASTQ files.
FASTQ files are transiently stored in password protected folders of our FTP server. Importantly, NGS raw data files must be transferred and archived by the costumers. We only transiently (8 weeks) store FASTQ files, after that the raw data are deleted.