Contact person
Szilárd Póliska
- E-mail: poliska@med.unideb.hu
- Phone: +36 52 411 717 /50216, 50210
Instrumentation
ABI SeqStudio Genetic Analyzer (Thermo Fisher Scientific)
This sequencing instrument is equipped with four capillary array and calibrated with BigDye 3.1 or BigDye 1.1 chemistry. This sequencing machine is suitable for generating 600 bp long sequencing reads.
Service
- Plasmid sequencing
- PCR product sequencing
We also provide general sequencing primers
| Primers |
Sequences |
|---|---|
|
T7 promoter |
GTAATACGACTCACTATAGGG |
|
T7 terminator |
GCTAGTTATTGCTCAGCGG |
|
T3 |
CAATTAACCCTCACTAAAGG |
|
SP6 |
TACGATTTAGGTGACACTATAG |
|
-21M13 Forward |
TGTAAAACGACGGCCAGT |
|
M13 Reverse |
CAGGAAACAGCTATGAC |
|
BGH Reverse |
TAGAAGGCACAGTCGAGG |
|
PGEX Forward |
GGGCTGGCAAGCCACGTTTGGTG |
|
PGEX Reverse |
CCGGGAGCTGCATGTGTCAGAGG |
Other oligonucleotide primers must be provided by the users (10 mM, volume: 2ml/reaction).
Sample types
- purified sequencing reaction products
- sequencing reaction products without purification
- DNA
- PCR product (amplified DNA): 30 ng for one sequencing reaction
- Plasmid: 300 ng for one sequencing reaction
Sequence results are provided by email. All sequence data are submitted to the users in ’seq’ and ’txt’ format. The DNA sequence chromatogram file ‘ab1’ can be open with the following software:
- 4Peaks (Macintosh OS X).
- Chromas software (Windows/DOS).
Fragment analysis
- DS-30 filter D dye set calibration: 6FAM, HEX, NED, ROX
- DS-33 filter G5 dye set calibration: 6FAM, VIC, NED, PET, LIZ
We can only sequence those samples which are compatible with the DS-30 or DS-33 calibration. Further information about the available fragment analysis kits or reaction improvement can be obtained from the Thermo Fisher Scientific’s representatives.