DNA/RNA quality control and quantitation - Genomic Medicine and Bioinformatics Core Facility | Department of Biochemistry and Molecular Biology

Contact person

Szilárd Póliska

• E-mail: poliska@med.unideb.hu
• Phone: +36 52 411 717 /50216, 50210

We provide quality control service using the Agilent Bioanalyzer system for both DNA and RNA and quantification using a Qubit fluorometer.

Instrumentation

Agilent BioAnalyzer 2100

RNA integrity analysis

• RNA 6000 Nano chip: 12 RNA samples can be analyzed; quantitative range range: 25 – 500 ng/ml; sample volume 1 ml.
• RNA 6000 Pico chip: 11 RNA samples can be analyzed; quantitative range range: 50 – 5000 pg/ml; sample volume 1 ml.

DNA quantitation and fragment size analysis:

• DNA7500 chip: 12 DNA samples can be analyzed; fragment size range: 35-10380 bp; quantitative range range: 0.5 – 50 ng/m; sample volume 1 ml.
• DNA1000 chip: 12 DNA samples can be analyzed; fragment size range: 15-1500 bp; quantitative range range: 0.5 – 50 ng/m; sample volume 1 ml.
• DNA HighSensitivity chip: 11 DNA samples can be analyzed; fragment size range: 50-7000 bp; quantitative range: 5 – 500 pg/ml; sample volume 1 ml.

Qubit fluorometer

This benchtop fluorometer is suitable for the quantitation of DNA using the highly sensitive and accurate fluorescence-based assays; concentration range: 0.1-100 ng/ml.