Contact person
Szilárd Póliska
- E-mail: poliska@med.unideb.hu
- Phone: +36 52 411 717 /50216, 50210
Real-time quantitative PCR (RT-QPCR)
Real-time quantitative PCR technique is suitable to assess gene expression via absolute or relative quantitation of mRNA transcripts, in addition, genetic alterations - polymorphism or copy number variations - can be detected with this method.
Our Core Facility contains three RT-QPCR equipment which can be used after training. On-line registration is needed in our web calendar (http://193.6.150.227/~cal/index.php) to reserve and using the PCR instruments.
Instrumentation
Roche LC480 (384) (Life Science Building, 3rd floor, room number: 3.043)
This instrument is installed with a 384-well PCR block. Please use the recommended Roche PCR plate (cat number: 05102430001 LightCycler® 480 Multiwell Plate 384, clear) for this equipment. If somebody apply a different kind of PCR plate, in that case, all service cost must be refunded upon instrument damage or malfunction.
Roche LC96 (96) (Life Science Building, 3rd floor, room number: 3.043)
This instrument is installed with a 96-well PCR block, please use the recommended Roche PCR plate (cat number: 05102413001 LightCycler® 480 Multiwell Plate 96, clear) for this equipment. If somebody apply a different kind of PCR plate, in that case, all service cost must be refunded upon instrument damage or malfunction.
QuantStudio 12K Flex (In Vitro Diagnostic Building, 3rd floor, room number: 3.16.4)
The QuantStudio real-time PCR (Thermo Fisher Scientific) instrument is equipped with four type of PCR blocks; default format: fast 96 well block. If you want to use a different type of block, please indicate it online, moreover, contact with Szilárd Poliska via email (poliska@med.unideb.hu).
- Fast 96 well block: it must be used with the Thermo Fisher 0.1 ml fast 96-Well plates (cat number: 4346907, MicroAmp™ Fast Optical 96-Well Reaction Plate, 0.1 mL);
- 384 well plate: it must be used with the Thermo Fisher 384-Well plates (cat number: 4309849, MicroAmp™ Optical 384-Well Reaction Plate with Barcode);
- TaqMan Array Cards: it must be used with 384-well TaqMan Array microfluidic cards; details: https://www.thermofisher.com/hu/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays/taqman-gene-expression/real-time-pcr-taqman-arrays.html.
- OpenArray: This format deliver TaqMan Assays in a more flexible, high-throughput real-time PCR plate. The OpenArray format is ideal for validating disease associations, pharmaceutical target profiling, as well as other studies requiring large sample sizes. Details: https://www.thermofisher.com/hu/en/home/life-science/pcr/real-time-pcr/real-time-openarray.html?SID=fr-openarray-main