Amino acids and biogenic amines
Our research group has developed an 11-minute LC-MS method for the simultaneous analysis of 33 amino acids and biogenic amines from complex biological samples. During sample preparation, we use AccQ-Tag derivatization reagents to convert amino acids and biogenic amines into stable fluorescent derivatives that can be identified and quantified using both chromatography and mass spectrometry. Using this validated method, we examined the depletion of individual amino acids in cell cultures during the culture period, as well as the amino acid and biogenic amine content of clinically relevant biological samples such as serum, tears, and saliva. Our results may contribute to the optimization of cell culture experiments, the enhancement of recombinant protein production efficiency, and, in clinical trials, we may shed light on changes in the amounts of individual amino acids and biogenic amines and the underlying molecular processes.
Water-soluble vitamins
Our research group has developed an LC-MS-based test method that enables the detection and quantitative determination of water-soluble vitamins. The developed LC-MS method allows for the simultaneous analysis of 13 vitamins (ascorbic acid, myo-inositol, choline, thiamine, pyridoxine, pyridoxal, nicotinamide, para-aminobenzoic acid, pantothenic acid, folic acid, cyanocobalamin, biotin, and riboflavin).
Estrogen forms
Our research group has developed a method for the chromatographic separation and targeted mass spectrometric detection and quantification of estrone (E1) and estradiol (E2) forms.
Metabolomic analysis of wines
Using shotgun and targeted mass spectrometry, our research group has examined the metabolite profiles of several different Tokaj wines. Based on the metabolite profiles, we have demonstrated differences between Furmint and Aszú wines using both shotgun and targeted analyses. In addition, our research group is also examining the metabolomic profile of wine vinegar derived from the further processing of wines.
Analysis of drug metabolites
Our research group has developed and validated a targeted mass spectrometry method for the analysis of two bioactive metabolites of the therapeutic drug azathioprine, which aids in the monitoring of therapeutic drug levels. From 2025, metabolite quantification using this method will be available as a test at the UD Clinical Center.