Samples:
- Protein concentration: 1-2 mg/ml
Volume:
- 400 ul for sedimentation velocity (SV) experiments
- 100 ul for sedimentation equilibrium (SE) experiments
Buffer:
- phosphate buffered saline (PBS) if possible
- ionic strenght: always use > 10 mM NaCl or other salt
- try to avoid the use of glycerol and sucrose
- low amounts of HEPES, Tris, DTT is allowed
Other requirements:
- protein sequence including any tags/extra residues after cleavage (.txt)
- protein mass corresponding to the sequence
- PDB code, if available
- composition of the buffer
Limitations:
- Size range: 100 Da - 108 Da
- KD: pM – mM
For further information :
Last update:
2022. 07. 18. 13:09